全文获取类型
收费全文 | 174篇 |
免费 | 16篇 |
国内免费 | 1篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 8篇 |
2016年 | 2篇 |
2015年 | 13篇 |
2014年 | 13篇 |
2013年 | 16篇 |
2012年 | 16篇 |
2011年 | 4篇 |
2010年 | 9篇 |
2009年 | 11篇 |
2008年 | 7篇 |
2007年 | 10篇 |
2006年 | 9篇 |
2005年 | 5篇 |
2004年 | 7篇 |
2003年 | 4篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 6篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 4篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有191条查询结果,搜索用时 328 毫秒
161.
Jonne Tikkanen Hannu-Ville Leskelä Siri T. Lehtonen Vesa Vähäsarja Jukka Melkko Lauri Ahvenjärvi Eija Pääkkö Kalervo Väänänen Petri Lehenkari 《Cytotherapy》2010,12(5):593-604
Background aimsCongenital pseudarthrosis of the tibia (CPT) caused by neurofibromatosis type 1 (NF1) is a refractory disease occurring in childhood. We present two cases that had failed all earlier treatment attempts and, as a last treatment attempt, the patients were chosen to receive mesenchymal stromal cell (MSC) transplantation prior to amputation.MethodsThe MSC from bone marrow (BM) were harvested from the iliac crest and cultured in osteoinductive medium for 3 weeks. The cultured MSC were injected in solution into BM canals of the tibia and around the resection line or bone defect in a 3-dimensional collagen sponge scaffold. After the MSC transplantation, the patients were monitored during a 10-month follow-up period. In both cases, bone formation at the pseudarthrosis site was observed and two of three treated bone defects healed. For clinical reasons not related to cell transplantation, such as new infection and pseudarthrosis and severe shortening of the leg, both extremities were finally amputated and bone samples were analyzed to evaluate MSC therapy effect and safety.ResultsMSC transplantation normalized bone remodeling, promoted bone resorption and improved the overall structure of bone. The number of osteoclasts in the cortical bone was 2-fold higher compared with the monitored situation before MSC transfer. In addition, the mineral content of the bone improved after transplantation. We could see no sign of aberrant bone formation or malignant transformation.ConclusionsOur data suggest that MSC transplantation is a possibility for treatment of CPT caused by NF1 in less severe cases without adjunct defects. 相似文献
162.
Males in polygyne populations of Solenopsis invicta are primarily sterile diploids and thought to not express the Gp-9 gene coding for a pheromone-binding protein affecting complex social behavior. We examined an aspect of the breeding system hitherto not considered--male Gp-9 genotypes in relation to sperm stored in queens. Four sites with varying frequencies of sympatric monogyne and polygyne colonies were sampled, including sexuals, workers, and broods from four colonies. Most queens were heterozygotes storing B sperm. Although predicted to be common, only 14 of 504 males were B or BB genotypes, suggesting strong selection. Increased frequency of polygyne colonies at each site paralleled increases in queens with b sperm (1.9-32.8%) and of noninseminated queens. The presence of both B and b sperm in 1.9-18.9% of queens, genotype profiles of colonies, and genotypes of offspring from individual queens suggest some frequency of multiple mating. The bb genotype, rather than an obligate, developmental lethal, was present in some queens and common in alates, workers, and brood. Selective mortality of sexuals may affect multiple aspects of the breeding system, including female-mediated dispersal, mating success, and gene flow. 相似文献
163.
《Journal of thermal biology》2002,27(5):423-428
- (1)The preferred temperatures of Macrobrachium acanthurus were determined for prawns acclimated to 20°C, 23°C, 26°C, 29°C and 32°C, and the final preferendum estimate was (29.5°C).
- (2)The critical thermal minima (CTMin) and maxima (CTMax) were 11.0°C, 12.1°C, 13.0°C and 14.8°C, and 34.2°C, 35.0°C, 36.1°C and 39.8°C, respectively.
- (3)The zone of thermal tolerance assessed using the CTMin and CTMax boundaries was 644°C2.
- (4)The acclimation response ratio was between 0.33 and 0.62.
- (5)To cultivate this species in the southeastern region of México it should be done in not <15°C (CTMin) during the winter and below 38°C in summer (CTMax).
164.
Effective water allocation among multiple jurisdictions is a key instrument to improve water use efficiency within the basin scale. To achieve equitable and reasonable water allocation, natural, socio-economic, and ecological conditions within a specific basin need to be systematically considered. Based on the main principles of equitable and reasonable water allocation that were defined by UN Watercourses Convention, an integrated multi-criteria decision making (MCDM) with bankruptcy rules (IMCDM-BR) under multiple hydrological constraints were proposed to allocate trans-jurisdiction water resources in Guanting reservoir basin (GRB), a shared basin between Zhangjiakou and Beijing in China. Projection pursuit (PP), as one of an effective MCDM approach, was employed to synthesize values of the related principles, which were ranked as weights for the corresponding water claims by relevant agents. Then, the weighted bankruptcy rules (BR) with multiple hydrological constraints were applied to allocate water among the related agents of the studying basin. The results of ordinary bankruptcy rules, bankruptcy rules with regular and hydrological constraints, and the proposed methods were compared and discussed. Among them, the proposed IMCDM-BR was recommended as an effective tool to support practical water allocation. Moreover, factors of equitable and reasonable water allocation were comprehensively considered. The results can thus be used for facilitating negotiation in trans-jurisdiction water allocation among agents within basins. 相似文献
165.
Vegetative, fruit, and floral characters of more than 200 herbarium specimens of Acacia constricta and closely related species from the Southwestern U.S. and Mexico were scored and analyzed by principal components analysis (PCA) and stepwise discriminant analysis (SDA). The results show that all of the 5 species of this group are distinct and form nonoverlapping groups in plots of the first three principal components. All taxa were found to be quite homogeneous as indicated by the tight clusters formed in the PCA plots. Acacia pacensis, whose relationship to the species of this group has been questioned, is confirmed to be more closely related to A. farnesiana and related species. Some specimens with a combination of characters from A. constricta, A. neovernicosa, and A. schottii were positioned intermediate to the putative parental species groups in the PCA and SDA plots, suggesting hybridization among these taxa. 相似文献
166.
167.
168.
Beyond biodiversity: individualistic controls of invasion in a self-assembled community 总被引:5,自引:0,他引:5
Recent experimental and simulation results, and competition‐based ecological theory, predict a simple relationship between species richness and the invasibility of communities at small spatial scales – likelihood of invasion decreases with increasing richness. Here we show data from 42 continuous years of sampling old field succession that reveal quite different dynamics of plant invasion. Contrary to experimental studies, when richness was important in explaining invasion probability, it was typically positively associated with species invasion. Invasion of several species had a unimodal response to resident species richness, which appeared to be a mixture of compositional influences and a richness effect. Interestingly, invasions by native and exotic species did not fundamentally differ. Control of species invasion in this system is individualistic, caused by a variety of community‐level mechanisms rather than a single prevailing richness effect. 相似文献
169.
170.